FastQCFastQC Report
Thu 2 Jun 2016
DRR025029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780995
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117430.24561832840235143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102380.21413952534984873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65880.1377955843919519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98500.051.3828661
GTATCAA174450.038.7461051
ATCAACG219450.030.439483
TCAACGC221950.030.132044
TATCAAC231450.029.105012
CAACGCA231500.028.9702175
AACGCAG240250.028.0129226
GTACATG194350.026.428981
TACATGG192150.025.296412
ACGCAGA270100.024.5163657
CGCAGAG271300.024.2166168
ACATGGG195850.023.5463813
GCAGAGT288850.022.1432749
AGAGTAC260550.020.5802510-11
TACCTGG36550.020.3211562
GAGTACT165850.020.31700712-13
CATGGGG111400.020.1238924
GTGGTAT46850.020.0799521
AGTACTT172100.019.48359912-13
CAGAGTA282000.019.37313510-11
Produced by FastQC (version 0.11.5)