FastQCFastQC Report
Thu 2 Jun 2016
DRR025028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273523
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92570.28278402198487684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76750.23445688330279033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.1332509348490907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.047.548771
GTATCAA97700.037.9251021
ATCAACG121250.030.2044663
TCAACGC121900.029.9658184
GTACATG93800.028.6740041
TATCAAC128850.028.5723462
CAACGCA129100.028.3305855
TACATGG91600.027.6156562
TACCTGG27000.027.5145152
AACGCAG135150.027.1981776
GTACCTG30300.025.7608721
ACATGGG96050.025.7456473
CGCAGAG151100.023.8261418
ACGCAGA152500.023.7629577
GTGGTAT29700.023.1148741
CATGGGG55450.022.9761124
TGGTATC29650.022.2009682
GCAGAGT160750.021.7227089
AGAGTAC147100.020.43198810-11
GAGTACA69250.019.6912521
Produced by FastQC (version 0.11.5)