FastQCFastQC Report
Thu 2 Jun 2016
DRR025028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273523
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103980.31763943616708973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91090.278262899023468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57730.176354343623063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59550.050.1578641
GTATCAA108550.040.9062081
ATCAACG133900.032.545233
TCAACGC134600.032.3685574
CAACGCA140950.030.9769975
TATCAAC141750.030.875562
TACCTGG27250.030.534792
GTACCGG3600.030.0519311
AACGCAG148300.029.568046
GTACCTG31400.028.012961
GTACATG95200.026.63171
ACGCAGA168950.025.6465077
CGCAGAG169600.025.3538638
TACATGG94100.024.9786282
ACATGGG94500.024.0769673
GCAGAGT178950.023.5301769
GTACGAC2103.852074E-822.3856093
GTGGTAT31600.021.2860971
TGGTATC30300.021.2552472
ACCTGGG38900.020.544123
Produced by FastQC (version 0.11.5)