FastQCFastQC Report
Thu 2 Jun 2016
DRR025027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410395
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97660.28635979116788524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.23786101023488485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48670.14271074171760162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65250.050.507291
GTATCAA115700.040.0239031
ATCAACG140300.032.6686863
TCAACGC141400.032.5138054
TATCAAC145450.031.449182
CAACGCA148750.031.1284585
AACGCAG156100.029.8107176
TACCTGG31700.027.5844232
ACGCAGA172400.026.63547
CGCAGAG173650.026.2259868
GTGGTAT31350.025.6433721
GTACCTG35400.025.6312981
GCAGAGT182600.024.2967229
TGGTATC31700.023.8768392
GTACATG101500.023.622221
TCTGTCG9100.023.2409538
TACATGG101550.022.8695282
TATAACG3400.022.123372
ACATGGG102650.021.7988073
TACAACG7500.021.3121782
Produced by FastQC (version 0.11.5)