FastQCFastQC Report
Thu 2 Jun 2016
DRR025027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410395
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111180.3260032928736994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91160.2673004153477823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65630.19244105154974717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.049.7301561
GTATCAA118150.040.00551
ATCAACG150600.030.6831053
TCAACGC152700.030.1951394
TATCAAC155350.029.8962292
CAACGCA158750.029.0443955
TACCTGG30350.028.8087882
AACGCAG165400.028.0187326
GTACCTG35250.025.4836481
TACAACG6100.025.4304982
ACAACGC6200.025.0203293
ACGCAGA187650.024.3941487
CGCAGAG187900.024.1608878
GTACATG99100.023.5868571
GTACCGG3250.023.153891
GCAGAGT198550.022.296859
GTGGTAT33450.022.2151031
TACATGG99500.021.9685122
ACATGGG99050.021.2615183
AGAGTAC183450.020.96831310-11
Produced by FastQC (version 0.11.5)