FastQCFastQC Report
Thu 2 Jun 2016
DRR025026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506636
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.37412691751016103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77510.3092192085328703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.19863274923044272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.057.6324271
TACCTGG29050.045.6417852
TATAACG2150.041.550362
GTATCAA95500.041.2088431
GTACCTG34150.039.6524541
ACCTGGG35050.036.7503553
GTACCGG2800.033.5846061
ATCAACG119750.032.4660153
TCAACGC120050.032.4221044
ACAACGC5700.032.1653823
CAACGCA125400.031.1513215
TATCAAC127350.030.717372
AACGCAG132400.029.749916
GTGGTAT20400.029.5017741
TGGTATC20900.027.6705932
GTCTCGC6200.027.3010391
GTACATG89950.026.7108731
ACGCAGA145150.026.6488087
GTACACG2305.456968E-1226.5756471
CGCAGAG145550.026.4453538
Produced by FastQC (version 0.11.5)