FastQCFastQC Report
Thu 2 Jun 2016
DRR025026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506636
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103870.414380069543404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87030.34719839657612833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60680.24207742967068216No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.1045624494342218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.062.0664521
TACCTGG28800.052.231312
GTACCTG34850.044.2659571
ACAACGC5550.044.0437053
GTATCAA97500.041.147481
ACCTGGG36300.040.5332263
TATAACG2950.036.6504442
GTGGTAT18700.032.6964451
ATCAACG122300.032.325333
GTACCGG2800.031.9149631
TCAACGC124200.031.710954
TGGTATC19700.031.2592492
TATCAAC128450.030.997222
CAACGCA130350.030.3950825
GTACACG3150.029.8619541
AACGCAG137300.028.9592046
GTATAAC7000.026.2038651
GTACAAC9250.025.9314751
ACGCAGA153050.025.8234837
CGCAGAG153700.025.744858
Produced by FastQC (version 0.11.5)