FastQCFastQC Report
Thu 2 Jun 2016
DRR025025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80340.24579947242144604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67600.20682156255526205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42120.12886574282289404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53700.053.92871
TACCTGG35200.041.535362
GTATCAA95700.040.282411
GTACCTG39350.037.8728561
ATCAACG119250.031.5743543
ACAACGC7050.031.3378433
TCAACGC121400.031.0534174
TATCAAC121950.030.9166762
GTGGTAT23950.030.425641
ATAACGC3900.030.1325423
CAACGCA127400.029.7754145
TCTGTCG11250.029.2437298
TATAACG3600.028.7289922
AACGCAG134450.028.561116
GTACCGG4450.028.524451
GTCTCGC11500.028.2075121
ACCTGGG53200.027.1260833
TAACGCA4450.026.407894
TACAACG8950.026.2631742
ACGCAGA146150.025.9197087
Produced by FastQC (version 0.11.5)