FastQCFastQC Report
Thu 2 Jun 2016
DRR025025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90860.27798531322146613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76620.23441816749976596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54300.16613033796968535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.055.3236771
GTATCAA99350.040.8120961
TATAACG3400.040.1016732
TACCTGG35550.039.411242
ACAACGC5900.034.2657173
GTACCTG41300.034.0541951
ATAACGC4250.032.0813373
ATCAACG130050.030.8015563
TAACGCA4600.030.6540034
TCAACGC130800.030.5805744
TCTGTCG11500.029.8328
TATCAAC135500.029.7708632
CAACGCA136650.029.4433985
GTCTCGC11100.028.8161161
TGGTATC23500.028.0093862
AACGCAG145050.027.9003246
GTGGTAT25150.027.1193661
GTACCGG3100.025.7950741
ACCTGGG53300.025.5808053
ACGCAGA160100.025.0408067
Produced by FastQC (version 0.11.5)