FastQCFastQC Report
Thu 2 Jun 2016
DRR025024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404180
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219990.6462349229476703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176020.5170701901779577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117160.3441651146531617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71100.2088608710467719No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.15933352525424624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45890.13480485755747346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.10495919722223854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122100.048.0138441
TACCTGG38850.045.741472
GTATCAA212700.037.9947931
TACAACG8950.037.2944832
TATAACG4500.036.5649382
ACCTGGG48100.036.258733
GTACCTG52100.034.921181
ACAACGC11550.031.746543
ATCAACG266000.029.9551733
TAACGCA5700.029.6900834
TCAACGC269450.029.536744
TATCAAC272650.029.4504322
CAACGCA280850.028.3541345
GTACAAC13950.027.6347371
AACGCAG290000.027.6171476
GTACATG210500.026.2422731
TACATGG209350.025.5776162
ATAACGC6650.025.4486433
GTGGTAT60700.024.706871
ACGCAGA323050.024.5426047
Produced by FastQC (version 0.11.5)