FastQCFastQC Report
Thu 2 Jun 2016
DRR025023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948344
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155960.39500104347544185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130350.33013840739307415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82470.20887237788804622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84300.055.3834951
GTATCAA157800.043.4717331
ATCAACG199500.034.1233253
TCAACGC201850.033.7480554
GTGGTAT34600.033.428561
TATCAAC207400.032.8932152
CAACGCA210200.032.4956745
AACGCAG219950.031.199676
TGGTATC36150.031.0840262
ACGCAGA244050.027.8669137
CGCAGAG246850.027.3209558
TACCTGG37000.027.1931632
GCAGAGT261100.025.1458729
GTACATG168250.024.8151321
TACATGG163800.024.110882
GTACCTG45850.022.6626991
ACATGGG172400.022.1434063
ACCTGGG45700.021.0893573
AGAGTAC248150.021.04137810-11
CAGAGTA256250.021.02735310-11
Produced by FastQC (version 0.11.5)