FastQCFastQC Report
Thu 2 Jun 2016
DRR025023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948344
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173360.4390701519421813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149120.37767732497472356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101420.25686718279866194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39560.10019390407725365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84950.059.4122541
GTATCAA159450.042.3908351
ATCAACG208350.032.285333
TCAACGC209100.032.1863024
GTGGTAT31450.031.5574231
TATCAAC215550.031.3381482
CAACGCA217200.031.029265
AACGCAG226250.029.7457896
TACCTGG35900.029.7232272
ACAACGC7050.028.6707063
TGGTATC33800.028.6493722
ACGCAGA257800.025.8316737
CGCAGAG260950.025.3937828
GTACATG164550.024.0401861
GCAGAGT275400.023.5494469
ACCTGGG44350.023.529813
GTACCTG47050.023.4935111
TACATGG160900.022.9923462
TATAACG4250.022.1210442
TATACCG3850.021.9732285
Produced by FastQC (version 0.11.5)