FastQCFastQC Report
Thu 2 Jun 2016
DRR025022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4756263
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125830.2645564385316792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.22502960832906002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65690.13811263170266236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82800.053.9495351
GTATCAA153500.040.710931
ATCAACG191900.032.0193753
TCAACGC194400.031.582094
CAACGCA201150.030.638825
TATCAAC201950.030.6160352
AACGCAG211450.029.29866
GTGGTAT38750.027.6667141
TGGTATC37350.027.3171982
ACGCAGA233300.026.3304867
CGCAGAG232900.026.1935378
TACCTGG39300.025.7224862
GTACATG139550.024.7657761
TCTGTCG11800.023.8978148
GCAGAGT250850.023.8687979
TACATGG137200.023.8518562
GTACCTG43250.023.809621
ACATGGG141300.022.1254313
AGAGTAC231600.021.114410-11
CAGAGTA246150.020.34364910-11
Produced by FastQC (version 0.11.5)