FastQCFastQC Report
Thu 2 Jun 2016
DRR025022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4756263
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141100.2966614756164661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123990.26068785515014625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80200.16861977565159875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.054.812471
GTATCAA157300.040.0438421
ATCAACG198750.031.2224853
TCAACGC202000.030.782194
TATCAAC207850.030.0826422
CAACGCA208800.029.8922525
AACGCAG220850.028.367386
GTGGTAT40350.026.6978231
TATAACG5200.026.218572
TGGTATC38800.026.1719672
GTACCGG4000.025.872991
ACAACGC6950.025.0275063
ACGCAGA248300.024.9839387
TACCTGG40150.024.9406852
CGCAGAG247650.024.7640768
GTACATG138050.024.2279741
ATAACGC6550.023.6849963
TACATGG134400.023.2614462
GTACCTG45300.023.2612861
GCAGAGT265150.022.6688189
Produced by FastQC (version 0.11.5)