FastQCFastQC Report
Thu 2 Jun 2016
DRR025021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365909
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93870.27888454500701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76910.22849696768391542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48540.14421067236220586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.050.919521
GTATCAA109950.038.9562721
TACCTGG33750.033.0148622
ATCAACG137300.030.8496633
TCAACGC138700.030.5369134
TATCAAC141550.029.9260772
GTACCTG38150.029.5781481
CAACGCA147050.028.8983965
TATAACG2500.028.2088992
AACGCAG153200.027.8585646
ATAACGC3400.026.2706533
ACGCAGA168400.024.9793037
CGCAGAG168450.024.720048
GTCTCGC10900.024.5868361
TACAACG7150.024.3293532
GTACATG89050.024.2344441
TCTGTCG10200.023.9601558
GCAGAGT177350.023.0283139
TACATGG90200.022.9340652
ACCTGGG49000.022.833743
Produced by FastQC (version 0.11.5)