FastQCFastQC Report
Thu 2 Jun 2016
DRR025021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365909
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105940.31474410033069816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90630.26925861632028675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64780.19245915442158418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.050.280531
GTATCAA117750.039.8624231
ACAACGC7250.031.7739643
TACCTGG32500.031.1005742
ATCAACG151300.030.7616483
TCAACGC153350.030.4328964
TATCAAC153850.030.2823432
CAACGCA159850.029.3130055
GTACCTG38150.028.2315671
AACGCAG167750.028.1566756
GTGGTAT31750.027.9970421
TACAACG8300.027.7543662
TATAACG3700.026.6827682
TGGTATC33150.025.95262
ACGCAGA188500.024.8826737
CGCAGAG189650.024.4584668
ATAACGC3400.023.5062483
GCAGAGT197350.023.0758659
GTACATG91350.022.8595921
TCTGTCG10400.022.594448
Produced by FastQC (version 0.11.5)