FastQCFastQC Report
Thu 2 Jun 2016
DRR025020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607488
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155080.18016870891948963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.14165573045236893No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA90250.10485056731998929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155000.046.98231
GTATCAA261050.040.8445321
ATCAACG341850.031.0091743
TCAACGC343950.030.860314
TATCAAC353800.030.0301572
CAACGCA391400.027.094425
AACGCAG402250.026.4309446
ACGCAGA417350.025.1246577
GTACATG394450.024.599841
CGCAGAG433900.024.0685758
TACATGG400100.022.853782
GCAGAGT447700.022.6124179
ACATGGG419550.021.120633
GTGGTAT80550.019.843941
GAGTACA292800.019.6528221
AGAGTAC409850.019.322510-11
CAGAGTA435100.019.26515210-11
GAGTACT223350.018.8748512-13
GTACTTT224100.018.54991114-15
AGTACAT297200.018.349162
Produced by FastQC (version 0.11.5)