FastQCFastQC Report
Thu 2 Jun 2016
DRR025020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607488
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184100.21388353954138536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149180.17331421199773966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98280.11417965380840496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156000.047.546921
GTATCAA270500.041.887541
ATCAACG365900.030.8725073
TCAACGC367800.030.7316254
TATCAAC379600.029.832952
CAACGCA399500.028.3048615
AACGCAG413550.027.3998896
ACGCAGA452950.024.7457187
CGCAGAG466600.023.8504418
GTACATG390600.023.6014731
GCAGAGT485050.022.2652729
TACATGG398100.022.0582122
ACATGGG420750.020.2671973
AGAGTAC448600.019.79484410-11
AGTACTT256250.019.41294712-13
GAGTACT259050.018.8402812-13
GTACTTT254500.018.82636514-15
CAGAGTA471950.018.75075710-11
GTGGTAT78150.018.7174531
GAGTACA293350.018.2782271
Produced by FastQC (version 0.11.5)