FastQCFastQC Report
Thu 2 Jun 2016
DRR025019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329860
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.20656557024938452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.182338458980198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.10272849468574041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106600.032.1960831
GTATCAA163750.031.7549321
ATCAACG184300.027.5729053
TCAACGC186200.027.315854
TATCAAC192500.026.5714652
CAACGCA193150.026.2839815
AACGCAG199950.025.5055876
ACGCAGA218500.023.0154367
CGCAGAG217550.023.0071228
GTACATG99750.021.9168151
TACATGG99550.021.34592
GAGTACT139850.021.33944112-13
GCAGAGT232800.021.1971829
AGAGTAC183200.020.5611510-11
ACATGGG100250.020.538653
TACAACG7050.019.3386522
AGTACTT150800.019.13545612-13
GTACTTT158350.018.8315214-15
CAGAGTA233000.017.29607210-11
CATGGGG58850.016.6143744
Produced by FastQC (version 0.11.5)