FastQCFastQC Report
Thu 2 Jun 2016
DRR025019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329860
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99070.22880647411232696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88340.2040250724041886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.1319673153404498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112000.032.376641
GTATCAA167600.029.3530391
ATCAACG196150.024.5657633
TCAACGC198950.024.2858664
TATCAAC202800.023.8993152
CAACGCA205200.023.4774515
AACGCAG212000.022.7682156
GTACATG94050.022.3534281
TACATGG95050.021.4156112
AGAGTAC192300.020.6876910-11
ACGCAGA234450.020.5266957
CGCAGAG234200.020.4079028
GAGTACT150450.020.24170512-13
ACATGGG96950.019.8806573
TATAACG3850.019.5368232
AGTACTT164100.018.93028612-13
GCAGAGT251850.018.5298379
GTACTTT172900.017.57267414-15
CAGAGTA250000.016.07275810-11
CCGTGCA8500.016.0340549
Produced by FastQC (version 0.11.5)