FastQCFastQC Report
Thu 2 Jun 2016
DRR025018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817864
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138650.49203936030979495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112080.3977480815255811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76490.27144674121959045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44880.1592695744010357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.13907697461623414No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.11065118827594234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.054.3285641
GTATCAA127100.042.9783361
TACCTGG31100.036.125912
TATAACG3500.034.9209942
TACAACG7600.034.0196952
ATCAACG164650.032.6878623
TCAACGC166700.032.3422784
TATCAAC167700.032.1522752
TAACGCA4000.031.7282814
GTACCTG36100.031.513541
ACCTGGG35950.030.8571573
CAACGCA176050.030.6240375
ATAACGC4350.030.2560023
AACGCAG182100.029.6814046
ACAACGC9450.026.859923
ACGCAGA201150.026.5886127
CGCAGAG202900.026.289338
GTGGTAT32350.025.8663251
GTACATG129250.025.7144871
TACATGG129800.025.2066922
Produced by FastQC (version 0.11.5)