FastQCFastQC Report
Thu 2 Jun 2016
DRR025018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817864
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151530.5377477408419994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127210.45144123350168774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102680.36438948082661193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.15770810798533924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38990.13836721715455397No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.12310743172842975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68550.057.8992841
GTATCAA127350.040.803891
TACCTGG28650.032.6481482
TATAACG3050.032.363062
ATCAACG173300.029.7806283
TCAACGC175500.029.4566944
TATCAAC176250.029.3355122
CAACGCA182250.028.3657045
GTACCTG33050.028.3152121
TGGTATC27950.027.9162042
AACGCAG190650.027.2391726
GTGGTAT28700.027.199721
ACCTGGG34100.026.4653073
ATAACGC4000.025.851923
ACAACGC6600.024.926093
TACAACG6500.024.586442
ACGCAGA215050.023.8636137
CGCAGAG216900.023.421748
GTACATG124250.023.3521561
TAACGCA3900.022.8958844
Produced by FastQC (version 0.11.5)