FastQCFastQC Report
Thu 2 Jun 2016
DRR025017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584033
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129430.3611294873680013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113730.3173240871387066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80530.2246910114945928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.042.943941
GTATCAA144550.035.3718341
ATCAACG183150.027.5171623
TCAACGC184900.027.2737774
TATCAAC188800.026.8434662
CAACGCA192500.026.1725775
AACGCAG201550.025.2072436
TATAACG3850.023.2014272
GTACATG112600.022.6415961
TACCTGG28350.022.221512
ACGCAGA228050.021.9883467
CGCAGAG229000.021.8144328
TACATGG116750.021.0201322
GTGGTAT48900.020.488821
AGAGTAC210050.020.47125610-11
GCAGAGT240550.020.1809259
ACATGGG118000.019.9206623
AGTACTT162950.019.79849812-13
GAGTACT158700.019.18863912-13
GTACCTG33900.018.3155691
Produced by FastQC (version 0.11.5)