FastQCFastQC Report
Thu 2 Jun 2016
DRR025016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631214
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112790.3106123737130337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99320.27351734158328317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56850.1565592113271209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72550.050.871941
GTATCAA132000.040.1417271
ATCAACG163150.032.098033
TCAACGC165500.031.669794
TATCAAC171900.030.5773642
CAACGCA173750.030.273015
AACGCAG181150.029.2938216
ACGCAGA199300.026.3405287
CGCAGAG200250.026.0270928
GTGGTAT35850.025.7047251
GTACATG110550.025.5175251
TGGTATC35400.024.7026632
TACATGG108500.024.3956722
GCAGAGT211550.024.0589169
ACAACGC8850.023.3716663
TACCTGG29550.022.7516382
ACATGGG112000.022.581073
TATAACG3700.021.601372
GAGTACT150700.021.31502712-13
AGAGTAC196100.021.14953210-11
Produced by FastQC (version 0.11.5)