FastQCFastQC Report
Thu 2 Jun 2016
DRR025016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631214
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122700.3379035220727834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108490.2987706039908416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.2017782482662823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76450.054.5062831
GTATCAA139300.040.144041
TATAACG3150.031.3375952
ATCAACG179100.030.9176643
TCAACGC180900.030.5794074
TATCAAC184650.030.0647472
CAACGCA188500.029.3963665
AACGCAG195100.028.4497096
GTGGTAT33150.026.8144321
TGGTATC33200.026.0517372
ACGCAGA222050.024.890287
CGCAGAG221600.024.8337578
ACAACGC7100.023.8342293
GCAGAGT231850.023.1683129
TACCTGG26250.023.1002862
ATAACGC4700.023.0031323
GTACATG108900.022.760041
GTACCGG3400.022.1325471
TACATGG105200.022.0733472
AGAGTAC218850.020.94768310-11
Produced by FastQC (version 0.11.5)