FastQCFastQC Report
Thu 2 Jun 2016
DRR025015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7842844
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151880.19365424073206097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120420.1535412409069975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127350.052.5328251
GTATCAA237700.043.3744771
ATCAACG303450.033.94233
TCAACGC307300.033.5476464
TATCAAC325650.031.5871142
CAACGCA348650.029.568515
AACGCAG361250.028.572776
ACGCAGA379550.027.0069647
CGCAGAG392700.026.0063618
GTGGTAT56350.024.9460641
GTACATG377700.024.807621
GCAGAGT411300.024.281659
TACATGG365200.024.1885432
ACATGGG387550.022.246293
TGGTATC61600.021.9798892
AGAGTAC395350.019.84650610-11
CAGAGTA409300.019.76717810-11
CATGGGG252600.019.6524454
GTACTTT208600.018.80103514-15
AGTACTT216500.018.14726412-13
Produced by FastQC (version 0.11.5)