FastQCFastQC Report
Thu 2 Jun 2016
DRR025015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7842844
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170890.21789289701541942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136480.17401850655195997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100070.1275940207404355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125300.053.525941
GTATCAA237400.043.5652661
ATCAACG323450.031.7397173
TCAACGC327800.031.327074
TATCAAC341150.030.1897952
CAACGCA356000.028.7927385
AACGCAG370950.027.6955076
GTGGTAT50200.026.7015821
ACGCAGA409250.024.724327
GTACATG367450.024.1401121
CGCAGAG420750.023.9699048
TACATGG355850.023.6191182
GCAGAGT442250.022.4007979
TGGTATC57250.022.2513792
ACATGGG382750.021.3816493
AGAGTAC423300.020.02297410-11
AGTACTT242850.019.22606712-13
CATGGGG250000.018.5547244
CAGAGTA438200.018.51632910-11
GTACTTT240000.018.40675714-15
Produced by FastQC (version 0.11.5)