FastQCFastQC Report
Thu 2 Jun 2016
DRR025014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4959713
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159410.32140972673217183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128460.25900692237635525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79110.15950519717572367No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.056.331541
GTATCAA182350.042.4972651
ATCAACG230850.033.3832053
TCAACGC233850.032.8738484
TATCAAC236000.032.65572
GTGGTAT43450.031.817581
CAACGCA244850.031.3154265
AACGCAG253050.030.407226
TGGTATC42900.030.2512862
GTACATG224800.027.0256291
ACGCAGA286800.026.4826587
CGCAGAG287200.026.3470548
TACATGG224100.026.1647552
ACATGGG229750.024.4767973
GCAGAGT307400.023.9735749
CAGAGTA299150.020.95040510-11
GAGTACT189200.020.81425712-13
CATGGGA102600.020.8055234
CATGGGG117700.020.6934
AGAGTAC289300.020.2909510-11
Produced by FastQC (version 0.11.5)