FastQCFastQC Report
Thu 2 Jun 2016
DRR025014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4959713
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181570.36608973140179685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149770.3019731182026057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.20597966051664682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95450.057.4631161
GTATCAA186400.044.7182041
ATCAACG245700.033.6178783
TCAACGC250900.032.931244
TATCAAC254350.032.496032
CAACGCA260550.031.7656765
GTGGTAT43050.030.8136061
AACGCAG271000.030.6095186
TGGTATC41750.029.5044782
ACGCAGA308150.026.6431487
CGCAGAG309650.026.4227568
GTACATG216250.026.0160681
TACATGG215650.024.9413342
GCAGAGT327650.024.4548289
ACATGGG221850.023.6699563
AGAGTAC312200.021.38251510-11
ATAACGC5800.021.0741023
TACCTGG40500.021.0119652
AGTACTT216150.020.66556712-13
GAGTACT208000.020.60544212-13
Produced by FastQC (version 0.11.5)