FastQCFastQC Report
Thu 2 Jun 2016
DRR025013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86690.23806326512734285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.21027228297151507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.12212104510569773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.055.6776961
GTATCAA114300.041.424711
ATCAACG142100.032.8880963
TGGTATC26500.032.8239022
GTGGTAT27350.032.492321
TCAACGC144200.032.376094
TATCAAC148450.031.6092852
CAACGCA152050.030.6702965
AACGCAG159600.029.3917036
CGCAGAG174350.026.4975058
ACGCAGA175750.026.3666537
GTACATG103850.024.2680321
GCAGAGT188450.023.816659
TACATGG100300.023.6262042
TCTGTCG9600.023.0091238
ACATGGG103000.022.138643
TACAACG8000.021.1581082
AGAGTAC177500.020.59940510-11
GAGTACT135800.020.29634512-13
CAGAGTA189650.020.02312910-11
Produced by FastQC (version 0.11.5)