FastQCFastQC Report
Thu 2 Jun 2016
DRR025013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641469
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94680.26000495953693414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.23133521114692998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55380.15208148140214842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.057.4565241
GTATCAA113750.041.2218481
ATCAACG144900.032.150383
TCAACGC146200.031.8262274
CAACGCA152650.030.5122495
TATCAAC153650.030.3505042
TGGTATC25650.030.0568392
GTGGTAT27600.029.6499421
AACGCAG159800.029.2642696
CGCAGAG178300.025.7779928
ACGCAGA179900.025.705827
ACAACGC5350.024.6028333
GTACATG98850.023.8366261
GCAGAGT191650.023.540959
TACATGG94900.023.3809722
TATAACG3007.2759576E-1221.9378262
GTACCTG25300.021.5635971
ACATGGG100400.021.4911583
AGAGTAC184250.020.82621210-11
GAGTACT144350.020.0714112-13
Produced by FastQC (version 0.11.5)