FastQCFastQC Report
Thu 2 Jun 2016
DRR025012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173920.4301774209785201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140900.34850505183919894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.2205549714159075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92350.058.137981
GTATCAA178450.043.734311
ATCAACG229300.033.5206343
TCAACGC230700.033.3567284
TATCAAC235750.032.82452
CAACGCA243050.031.6973325
AACGCAG251550.030.7174156
GTGGTAT39150.030.262131
TGGTATC38700.029.7628942
ACGCAGA276100.027.7293937
CGCAGAG277950.027.3246758
GCAGAGT294700.025.3091229
GTACATG192750.025.0499461
TACATGG188050.024.5003762
ACATGGG195800.022.617093
CAGAGTA289900.021.51310210-11
GAGTACT191000.021.48013712-13
AGAGTAC277200.021.01520310-11
TAACGCA3900.020.4911674
GTACTTT201250.020.23470514-15
Produced by FastQC (version 0.11.5)