FastQCFastQC Report
Thu 2 Jun 2016
DRR025012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194030.4799179219897784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162000.4006942398718965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111780.27647902551160863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.10885526849853189No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95150.058.7369271
GTATCAA179850.042.6102641
ATCAACG234200.032.5622863
GTGGTAT37950.032.3543241
TCAACGC237250.032.2137834
TATCAAC243900.031.248772
CAACGCA246500.031.004955
TGGTATC38200.030.401522
AACGCAG258650.029.6211916
TATAACG3650.027.05132
GTACATG191600.026.2719231
ACGCAGA293800.025.9621357
CGCAGAG293150.025.9068378
TACATGG187500.025.8033312
GCAGAGT312800.023.9187959
ACATGGG195800.023.6523653
GAGTACT206100.020.8075312-13
AGAGTAC296250.020.76558710-11
AGTACTT207050.020.53047412-13
CAGAGTA310900.020.42952510-11
Produced by FastQC (version 0.11.5)