FastQCFastQC Report
Thu 2 Jun 2016
DRR025011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360503
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105440.31376255280831467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87970.26177628765693706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.16583826885439473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61600.056.6334761
GTATCAA115300.041.6746181
ATCAACG143000.032.9753233
TCAACGC144450.032.6428574
TATCAAC148900.031.889732
CAACGCA151050.031.4636675
AACGCAG157250.030.457756
GTGGTAT26050.029.960591
TGGTATC26350.028.9040412
ACGCAGA172600.027.4754817
CGCAGAG173700.027.220318
GCAGAGT184600.025.2303899
TACCTGG28550.025.0300462
GTACATG103150.023.7475361
GTACCTG31900.023.5819281
TACATGG101950.022.4577332
CAGAGTA181300.021.9048810-11
ACATGGG103700.021.4440363
GAGTACT130800.021.21623212-13
AGAGTAC176400.021.03466210-11
Produced by FastQC (version 0.11.5)