FastQCFastQC Report
Thu 2 Jun 2016
DRR025011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360503
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116450.3465255052591829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100030.29766377235788805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66310.19732165095522905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.055.344661
GTATCAA116450.043.74691
ATCAACG148400.033.835753
TCAACGC150550.033.281174
TATCAAC153600.032.6912422
CAACGCA157400.032.071685
AACGCAG165050.030.7840566
GTGGTAT25250.029.6206151
ACGCAGA188700.026.847937
CGCAGAG188400.026.7410138
TGGTATC27550.025.9402052
TACCTGG30200.025.5322042
GTACCTG32000.025.2834761
GCAGAGT202250.024.607729
ACAACGC6650.023.330863
GTACATG104400.023.159061
TACATGG103250.022.2674352
AGAGTAC189850.021.27645510-11
AGTACTT144250.020.6881612-13
CAGAGTA196550.020.38380610-11
Produced by FastQC (version 0.11.5)