FastQCFastQC Report
Thu 2 Jun 2016
DRR025010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176458
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87820.27647146601655054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73420.23113795302818424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46420.14613761617499743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.057.421971
GTATCAA97050.045.203951
ATCAACG123600.035.0720943
TCAACGC125600.034.6242874
TATCAAC128450.033.8597872
CAACGCA132400.032.7379265
AACGCAG138700.031.4812326
GTGGTAT20100.029.7096771
ACGCAGA151950.028.3007977
CGCAGAG151700.028.2544968
CGCGCGA350.00884363526.85424290-91
TGGTATC22500.025.7039092
GCAGAGT163500.025.6691789
GTACATG84050.023.6083161
TACATGG83250.022.9872382
AGAGTAC156400.021.42533510-11
ACATGGG85750.020.9997373
CAGAGTA163000.020.80288710-11
GTACCTG25100.020.6067371
TACCTGG24050.020.5281982
Produced by FastQC (version 0.11.5)