FastQCFastQC Report
Thu 2 Jun 2016
DRR025010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176458
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98450.3099364134517126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.2764399844103086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59630.1877248180205751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.058.409681
GTATCAA109100.041.5610771
GTGGTAT23800.034.7832571
TGGTATC23950.032.1944732
ATCAACG141700.031.7188433
TCAACGC144000.031.0070534
TATCAAC145650.030.9241682
CAACGCA149650.029.8677965
AACGCAG156250.028.7866616
ACAACGC5150.027.3869293
ACGCAGA174750.025.5484647
CGCAGAG174150.025.5285438
GCAGAGT183900.023.6895269
AGAGTAC178150.021.40725910-11
TCTGTCG8150.021.3354848
GTACATG81450.021.309331
TACATGG80800.020.4820922
GAGTACT143500.020.43577212-13
CAGAGTA186400.020.40935710-11
GTACCTG25150.020.1985531
Produced by FastQC (version 0.11.5)