FastQCFastQC Report
Thu 2 Jun 2016
DRR025009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670956
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136320.24038275028055237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113520.20017788887799515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69190.12200764738784783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.054.0862121
GTATCAA183350.042.5988771
ATCAACG226350.034.191383
TCAACGC229650.033.7389264
TATCAAC239400.032.3292772
CAACGCA241800.032.0988245
AACGCAG253050.030.8335486
ACGCAGA282800.027.222597
CGCAGAG281100.027.0695448
GTACATG213500.026.275561
TACATGG206900.025.1808012
GTGGTAT46050.024.7112751
GCAGAGT305350.024.2882569
ACATGGG214850.023.4818153
TGGTATC46500.023.4598542
AGAGTAC283500.020.6397110-11
GAGTACT185700.020.30807112-13
CAGAGTA301900.020.1679410-11
AGTACTT190600.019.62572712-13
CATGGGG120200.019.4007134
Produced by FastQC (version 0.11.5)