FastQCFastQC Report
Thu 2 Jun 2016
DRR025009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670956
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154400.27226450002433455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.2302433663742057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86380.15231999684003897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105950.055.2745741
GTATCAA193750.040.86011
ATCAACG247000.031.725213
TCAACGC251000.031.2521254
TATCAAC260550.030.2212582
CAACGCA262100.029.9644555
AACGCAG275400.028.6019426
GTGGTAT45900.027.362491
TGGTATC46250.025.7156562
ACGCAGA309750.025.1855587
CGCAGAG310150.025.0015538
GTACATG213500.023.7507821
GCAGAGT333800.022.8641199
TACATGG208750.022.4746482
ACATGGG217550.020.8513453
GAGTACT209200.020.58854112-13
AGAGTAC314950.020.173910-11
AGTACTT209250.019.85370612-13
GTCGTAC3800.019.805851
CAGAGTA331600.019.74909810-11
Produced by FastQC (version 0.11.5)