FastQCFastQC Report
Thu 2 Jun 2016
DRR025008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519557
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180810.5137294267431952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144600.41084716059435883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95500.2713409670592066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40030.1137359048312046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.11120717749421305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85150.054.8345681
GTATCAA167050.043.7978671
TATAACG3750.036.3611872
ATCAACG209750.034.430893
TCAACGC213000.033.8820154
GTGGTAT33500.033.265451
TATCAAC221150.032.678212
TACCTGG26800.032.632432
CAACGCA225200.032.1871385
TGGTATC34800.031.7511842
AACGCAG236100.030.87646
GTACATG190250.030.1526181
TACATGG185200.029.399432
ACGCAGA260100.027.7181997
CGCAGAG258900.027.6829038
ACATGGG192750.027.1005843
GTACCTG34850.026.310051
GCAGAGT269300.026.1600829
ACCTGGG32200.025.844993
TAACGCA5900.023.906154
Produced by FastQC (version 0.11.5)