FastQCFastQC Report
Thu 2 Jun 2016
DRR025008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519557
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202050.5740779308304994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164790.4682123346773472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117140.33282597781482154No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44520.12649319218299349No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.107996546156235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37930.10776924482257283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.058.7545281
GTATCAA176000.042.6972161
TACCTGG27700.039.2158432
ATCAACG226250.032.9596253
TCAACGC230050.032.4454
ACCTGGG32800.032.396813
TATCAAC238500.031.3303242
CAACGCA241150.031.0295375
GTACCTG36050.030.4129071
GTGGTAT34850.030.2449251
AACGCAG252750.029.6971516
TATAACG3050.029.29432
GTACACG2750.029.0886481
TGGTATC36200.028.318962
GTACATG195200.027.6738361
TACATGG190900.027.4908162
CGCAGAG280300.026.2890328
ACGCAGA282800.026.2065667
ACATGGG198850.025.7259223
GCAGAGT297000.024.3361329
Produced by FastQC (version 0.11.5)