FastQCFastQC Report
Thu 2 Jun 2016
DRR025007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355724
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68180.28942269977297846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.24574186110087598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.14492359885962872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.046.702981
GTATCAA78350.039.06651
ATCAACG94450.032.0082783
TCAACGC95250.031.7861084
TATCAAC98400.030.7240472
CAACGCA99450.030.5829285
AACGCAG102200.029.8941826
TACCTGG18150.029.0137542
ACGCAGA113650.026.6744887
GTACCTG20650.026.639641
ACAACGC5400.026.1204593
CGCAGAG115200.026.1115888
GCAGAGT122700.023.9792489
GTACATG58750.023.2088011
GTGGTAT24050.023.0690361
TCTGTCG7000.022.8289898
TACAACG5850.022.504262
TGGTATC23700.022.4177822
GTACGGC2103.8638973E-822.37851194
TACATGG58450.021.960422
Produced by FastQC (version 0.11.5)