FastQCFastQC Report
Thu 2 Jun 2016
DRR025007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355724
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79020.33543827714961516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.28836145490728116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.19819809111763517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.051.5491261
GTATCAA82350.038.5541531
ATCAACG104100.030.074993
TCAACGC105400.029.6098164
TATCAAC107750.029.0998362
CAACGCA109250.028.6093795
AACGCAG113800.027.5888276
TACCTGG19650.025.8370692
GTACCTG21400.025.0565991
ACGCAGA129300.024.2800377
CGCAGAG130300.024.021058
GTCTCGC7350.023.6780221
TAACGCA2803.6379788E-1223.5005634
GTGGTAT23400.023.1160161
GTACATG56050.023.0774671
TATAACG2504.3655746E-1022.5643712
TGGTATC22700.022.1584782
CCGTAGA3007.2759576E-1221.9375842
ATAACGC3007.2759576E-1221.9375843
TCTGTCG6000.021.9315328
Produced by FastQC (version 0.11.5)