FastQCFastQC Report
Thu 2 Jun 2016
DRR025006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3553766
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85140.2395768320142632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75660.21290090568709363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.11964772019316973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.056.380281
GTATCAA112150.040.543561
ATCAACG135350.032.965053
TCAACGC136800.032.647714
TATCAAC143050.031.2248132
CAACGCA144550.030.9913965
AACGCAG151250.029.7382666
GTGGTAT26800.027.1951581
ACGCAGA166550.026.8073337
CGCAGAG166400.026.6337938
TGGTATC26300.025.2073672
GCAGAGT180000.024.0731589
GTACATG93050.021.7293531
GAGTACT130800.021.1264212-13
CAGAGTA180500.021.02519210-11
TACATGG90600.020.6028082
AGAGTAC168100.020.56314810-11
ACATGGG91100.019.611513
GTACTTT140650.019.44723714-15
AGTACTT135650.018.60419312-13
Produced by FastQC (version 0.11.5)