FastQCFastQC Report
Thu 2 Jun 2016
DRR025006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3553766
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90280.25404036168954286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84940.23901404875841573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.14483227089234352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.055.323961
GTATCAA118700.039.031331
ATCAACG149150.030.8570883
TCAACGC151400.030.3908124
TATCAAC154550.029.7806112
CAACGCA157150.029.338655
AACGCAG163700.028.1639546
GTGGTAT30850.027.4438611
TGGTATC31350.025.6435742
CGCAGAG180450.025.3145648
ACGCAGA181500.025.2457947
GTACATG96850.023.2628921
GTACCGT2253.1868694E-922.9764776
GCAGAGT195650.022.7713899
TACATGG93700.022.628562
ACAACGC5900.021.5133113
TCTGTCG9000.020.887128
GAGTACT140050.020.87220612-13
ACATGGG96450.020.763613
CTGTCGC10650.020.7400289
Produced by FastQC (version 0.11.5)