FastQCFastQC Report
Thu 2 Jun 2016
DRR025005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544138
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198920.5612648265953527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151140.42645066303851603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104130.2938091011128799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48250.13614029702003705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.11582506098803151No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.11088733000803015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95850.055.0407681
GTATCAA188800.042.8859371
TACCTGG29700.038.3094442
ATCAACG238150.033.677883
TCAACGC242550.033.1827744
TGGTATC38850.033.038372
ACCTGGG34150.032.9020923
TATCAAC253400.031.8574242
GTGGTAT41450.031.6492521
CAACGCA254500.031.6422625
GTACCTG37950.030.4794461
AACGCAG267200.030.3601726
GTACATG220900.028.6292741
TACATGG213000.028.253832
ACGCAGA290700.027.5340127
CGCAGAG290100.027.4933628
TACAACG7700.026.2557662
ACATGGG223550.026.1614633
GCAGAGT303200.025.7784529
CATGGGA129650.023.279454
Produced by FastQC (version 0.11.5)