FastQCFastQC Report
Thu 2 Jun 2016
DRR025005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544138
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224250.6327349555801721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179900.507598744744138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135180.3814185565009037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46620.1315411533072358No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44300.12499513280803401No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41690.117630859746432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36890.10408736905842832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100500.060.0506171
GTATCAA195750.042.7735371
TACCTGG29750.039.5072672
GTGGTAT37350.032.4927141
ACCTGGG35750.032.4798663
TGGTATC37200.032.3534772
ATCAACG259200.032.1563723
GTACCTG37050.032.1210141
TCAACGC263200.031.697584
TATCAAC267950.031.0909442
TATAACG4100.030.9602072
CAACGCA274600.030.4501235
AACGCAG286900.029.1929876
GTACACG3200.026.4593661
GTACATG203900.025.9994161
ACGCAGA321250.025.893747
CGCAGAG321450.025.7460448
TACATGG198400.025.5921062
GTACCGG2051.0732037E-925.2403721
TAACGCA5550.024.559454
Produced by FastQC (version 0.11.5)