FastQCFastQC Report
Thu 2 Jun 2016
DRR025004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724578
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104530.22124727330144617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90270.1910646834489768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.11258148346794147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.054.3429371
GTATCAA134050.043.246791
ATCAACG166000.034.5526853
TCAACGC167850.034.086384
CAACGCA175050.032.708115
TATCAAC175350.032.630872
AACGCAG182300.031.5064666
GTGGTAT30350.030.518641
ACGCAGA206500.027.6994937
CGCAGAG209550.027.1614678
TGGTATC34050.025.8207192
GCAGAGT224800.024.5032339
GTACATG129100.024.36451
TACATGG132400.022.5136092
TACCTGG36950.021.6310862
ACATGGG132850.021.2687973
GTACCTG42500.020.9088691
AGAGTAC210200.020.7492710-11
CAGAGTA219800.020.34551210-11
GTACCGG4400.020.3029211
Produced by FastQC (version 0.11.5)