FastQCFastQC Report
Thu 2 Jun 2016
DRR025004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724578
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118170.2501175766385908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105680.22368135312825824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70230.14864819672783475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77250.052.739121
GTATCAA137750.040.6755681
ATCAACG172200.031.999013
TCAACGC175100.031.4613824
TATCAAC181500.030.620052
CAACGCA182550.030.3319135
AACGCAG192250.028.9968016
ACGCAGA217350.025.536287
CGCAGAG217250.025.4398738
GTGGTAT36050.023.6203731
GTACATG129350.022.9496671
GCAGAGT237050.022.839159
TACATGG130700.021.512492
TGGTATC37850.020.993552
ACATGGG131500.020.5581553
AGAGTAC221350.020.5099710-11
GAGTACT163400.020.3345612-13
CAGAGTA232600.019.79074510-11
TACCTGG38150.019.596012
AGTACTT169800.019.51276612-13
Produced by FastQC (version 0.11.5)