FastQCFastQC Report
Thu 2 Jun 2016
DRR025003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650065
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96960.26563910505703325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83850.22972193645866582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.13109355586818316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.056.845041
GTATCAA109400.041.479921
ATCAACG135950.033.1334423
TCAACGC137350.032.8285984
TATCAAC142100.031.7342762
CAACGCA143600.031.3937535
AACGCAG148100.030.3730476
ACGCAGA163150.027.396887
CGCAGAG162850.027.1583618
TGGTATC24700.027.0330732
GTGGTAT26850.026.9714531
GTACATG89950.025.407561
GCAGAGT176600.024.6712279
TACATGG89300.023.853432
ACATGGG92050.022.1690043
AGAGTAC166350.021.62838610-11
CAGAGTA173550.021.47584510-11
GAGTACT129900.021.43445812-13
TCTGTCG10700.021.0843018
TACCTGG28150.020.8802492
Produced by FastQC (version 0.11.5)